Milk Culturing

  1. Before plating, clean inside the hood (protective incubator) with Bac Down and wipe down. Spray with alcohol and wait to dry.
  2. Place vortex, loop sticks and empty jar in the hood
  3. Milk samples were thawed out and the tubes were placed on a vortex to shake up the contents .
  4. Each plate was split into 4 sections which correlated with each teat.
  5. Moving counter clockwise on the plate and using a separate loop stick for each milk sample, in a swift movement, swipe milk sample onto its identified section.
  6. Plates are placed in incubators for 24 hours. When revisiting plates if there two types of growth (bacteria) it identifies infection.  
Milk tubes are placed on vortex which shake up the contents
Using the a loop stick, dip into milk tube
Smear loop stick onto plate in a swift motion