These two images show positive results for Rumen fluke from individually isolated eggs from faecal samples and using the ITS2 Flks primers and PCR settings from the 2016 paper. These results mean I have sufficient amounts of positive DNA pool of rumen fluke to begin my samples from the better farm project.

My next step is trying to get my species specific Primers to work by figuring out their specific PCR settings.  My course coordinator designed species specific primers  for me, however, I need to know the PCR settings for them that will work to continue.