Terrible Tetranychus

I think its time to talk about mites. The Tetranychus species are commonly referred to as red spider mites. The different species are difficult to tell apart as they are all a rusty red in colour. The red spider mites have a world wide distribution but are most common in the tropics and subtropics. There any many crops that are hosts to the spider mites, these include: okra, papaya, sweet potato, tomato, eggplant, beans, taro, cucumber, squash and of course cassava which is the host crop I am studying.

Yellow spots present on this cassava plant indicate a high volume of mites

Symptoms & Life Cycle

Spider mites are common plant pests. They have needle-like mouthparts and use them to suck juice from the leaves. This destroys the cells, and the leaves show a characteristic white to pale yellow speckling, often along the sides of the main veins When infestations are severe, the speckling is seen all over the leaf. Some species of red spider mite such as the two-spotted mites make webs (like spiders) on the under and on the upper surface during an infestation as the infestation advances, the leaves turn yellow and die prematurely. The eggs are round and relatively large in comparison to the size of the adult; they are laid in the webbing near the veins, on the underside of leaves. The webs allow the mites to travel from infested to non-infested leaves; also, the webs are caught by the wind and help the mites to disperse by this method also. The eggs hatch 3 days after being laid producing larvae that have six legs and are colourless. From these, nymphs develop, which have eight legs; they moult once and within a few days become adult. The adults are about 0.5 mm long, with males smaller than females, and narrower towards the back end. Each female lays about 100 eggs. Under tropical conditions the life cycle takes only 7-10 days depending on temperatures. Populations develop rapidly, especially during periods of drought when damage can be considerable. The adults live for 2-4 weeks.

Impact

The extent of the damage caused by mites often depends on rainfall. When rainfall is low, mite populations are high and reduce crop yields. On cassava, for instance, yellowing of the leaves and early maturity of plants occurs and the size of the root tuber is reduced. The overall crop yield is also reduced. Damage is particularly severe during droughts and it is thought that outbreaks will increase with climate change.

Mites present on the veins of this leaf

Management

There are different ways to manage mites:

CULTURAL CONTROL

  • A regular spaying of leaves with water can keep spider mites in check.
  • Check that cuttings, “tops”, and other kinds of planting materials are free from mites infestation before planting in a new garden.
  • Weed: remove plants that are common hosts of spider mites, e.g., wild Amaranthus 
  • The problem with cultural control is that it is very time consuming especially if you are managing a large area of land or many different fields which is the case where I am carrying out my research.

CHEMICAL CONTROL

  • Pesticides can be used but they should be applied carefully, rotating between different chemical groups, to prevent resistance developing to any one of them.
  • Not all insecticides kill mites, and those that do may not kill all the stages. Eggs are particularly resistant to pesticides and so, too, are larvae and nymphs, especially when moulting, as they do not feed. More than one application is needed at 5-10 days apart.
  • Aside from the difficulties associated with pesticide applications there is also a health concern. Many people in Vietnam, especially where I am based, have fears surrounding pesticides and their potential

NATURAL ENEMIES
Predatory mites keep populations of spider mites in check, as do ladybirds, beetles and lacewing larvae. Managing mites requires preserving natural enemies; in most cases this means doing nothing to harm them, which means not using pesticides. The Project I am involved in is interested in using predatory mites as a biological control which if successful could eventually lead to farmers being taught how to raise predatory mites for release into their fields to control red spider mite numbers.

Hungry Hungry Hippos

I don’t know if you ever played the board game ”hungry hungry hippos” as a child but it was one of my favorites to play during free time in primary school and I was reminded of that during my research this week. The object of the games is to get your hippo to eat more marbles than anyone else. This week I have been mainly focused on my leaf dish experiments where I place eggs of the red spider mites on a leaf, placed on a sponge soaked in water. I have a leaf with 5, 10, 15, 20,25 and 30 eggs and I repeat these 5 times and once I have them all set up with the correct number of eggs I place one adult female predatory mite on each. Now this is where ”hungry hungry hippos” comes in. The predatory mites have been starved for 24 hours and once placed on the dish they begin to feast. My field supervisor here speaks very little English and our main forms of communication are hand gestures, sound effects and google translate. So earlier in the week during our first round of this experiment he pointed at his stomach, pointed at the dish and said ”hungry hungry”. I couldn’t help but think of the board game I loved so much when I was younger, watching the mites scurry around the dishes eating as many eggs as they can. I nearly find myself rooting for predatory mites as I watch under the microscope I am already looking forward to repeating this experiment with different life stages of the red spider mites.

my leaf dish experiments or as they have now been nicknamed, ”my hungry hungry hippos”

Research Refresh

Just a quick update about where I am at with my research. I am still based out in the field and I will probably be here for the next two weeks. I have 5 rounds of my sampling done and only one left to do next week. so I have collected and looked at 3000 leaves to date and with some leaves having 50-60 mites I can’t even begin to estimate how many mites I’ve counted. At each site I record the temperature, humidity and soil moisture to see if there is a difference in these between mono-crop and inter-crop fields. I am yet to sit down and take a proper look at my results to see if there is anything interesting happening and I am looking forward to being back in the lab in Hanoi with some time set aside for statistical analysis.

As well as collecting leaves I am also carrying out leaf dish experiments to check the predation capacity of predatory mites on red spider mites. The Adult female predator mites are starved for 24 hours and one is transferred onto a leaf with 5 red spider mites, one to a leaf with 10 red spider mites and so on increasing in 5s until a maximum of 30 mites is reached. This is repeated 5 times to get an accurate idea of what the predation capacity is. So far I have looked at the predation capacity of the adult female predator mites on spider mite eggs and I will probably repeat this experiment using a different life stage of the red spider mites to get a clearer idea of the predation capacity. In the lab I keep leaves that I have collected in water, these leaves contain my red spider mites. I also have a single leaf dish set up that contains the predatory mites as these have to be isolated to be starved in order for the predation experiment to work.

Leaves in the lab with red spider mites
Leaf dish with the isolated predatory mites.

I have also finished carrying out my soil infiltration experiments that I talked about in a previous post. The last thing left to do is to start collecting mites to bring back home to the lab in NUI Galway for DNA analysis. So it really is all go go go here trying to get everything done in the next 4 weeks or so. After a brief plateau I feel refreshed and ready to get all this work completed.

soil infiltration test on the slope