{"id":58,"date":"2019-04-18T13:13:46","date_gmt":"2019-04-18T13:13:46","guid":{"rendered":"http:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/?p=58"},"modified":"2019-04-18T13:13:47","modified_gmt":"2019-04-18T13:13:47","slug":"pcr-2","status":"publish","type":"post","link":"https:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/2019\/04\/18\/pcr-2\/","title":{"rendered":"PCR"},"content":{"rendered":"\n<ul class=\"wp-block-gallery columns-1 is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\"><li class=\"blocks-gallery-item\"><figure><img loading=\"lazy\" decoding=\"async\" width=\"768\" height=\"1024\" src=\"http:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/wp-content\/uploads\/sites\/61\/2019\/04\/PCR-18.4-1-768x1024.jpg\" alt=\"\" data-id=\"59\" class=\"wp-image-59\" srcset=\"https:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/wp-content\/uploads\/sites\/61\/2019\/04\/PCR-18.4-1-768x1024.jpg 768w, https:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/wp-content\/uploads\/sites\/61\/2019\/04\/PCR-18.4-1-225x300.jpg 225w, https:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/wp-content\/uploads\/sites\/61\/2019\/04\/PCR-18.4-1.jpg 960w\" sizes=\"auto, (max-width: 768px) 100vw, 768px\" \/><\/figure><\/li><\/ul>\n\n\n\n<p>This PCR was carried out on 18\/4\/19<\/p>\n\n\n\n<p>The first two samples were 100 isolated eggs, stored overnight in ethanol, and broken with a sonicbeater. Then using the QIAamp stool kit I washed and eluted the sample and recorded the DNA concentration using a nanodrop. The other 5 samples are from faecal samples of cattle I collected on the teagasc farm. The primers used were the ITS2 FLKS from the 2016 paper. <\/p>\n\n\n\n<p>This PCR now shows ive working primers and PCR settings.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>This PCR was carried out on 18\/4\/19 The first two samples were 100 isolated eggs, stored overnight in ethanol, and broken with a sonicbeater. Then using the QIAamp stool kit I washed and eluted the sample and recorded the DNA concentration using a nanodrop. The other 5 samples are from faecal samples of cattle I &hellip; <a href=\"https:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/2019\/04\/18\/pcr-2\/\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">PCR<\/span> <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":64,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-58","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/wp-json\/wp\/v2\/posts\/58","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/wp-json\/wp\/v2\/users\/64"}],"replies":[{"embeddable":true,"href":"https:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/wp-json\/wp\/v2\/comments?post=58"}],"version-history":[{"count":1,"href":"https:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/wp-json\/wp\/v2\/posts\/58\/revisions"}],"predecessor-version":[{"id":60,"href":"https:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/wp-json\/wp\/v2\/posts\/58\/revisions\/60"}],"wp:attachment":[{"href":"https:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/wp-json\/wp\/v2\/media?parent=58"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/wp-json\/wp\/v2\/categories?post=58"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.plantagbiosciences.org\/people\/eoin-dunne\/wp-json\/wp\/v2\/tags?post=58"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}